The Co-Isolation of Lactic Acid Bacteria (LAB) and A Related Pathogenic Strain from Pangasius Nasutus

Catfish species Pangasius nasutus, or locally known as Patin Buah is one of a popular aquaculture product in Malaysia. Probiotic strain an attractive alternative to conventional antibiotics managing diseases. can be source bacterial developing suitable probiotic useful rearing. This study highlights on the isolation LAB strains from selected tissues P. nasutus which are heart, stomach, and intestines. These were subjected morphological, biochemical, genotypic characterisations. Three different isolated fish tissues, H-Hn heart; S-Hn stomach; I-Sk intestine. Biochemical characterisation consistent with ribosomal rRNA sequencing, (acc. No. MW504962) highly similar (100%) Lactococcus lactis, both (MW504964) (MW504963) (99%) garvieae. All appeared non-spore forming, non-motile, Gram-positive coccus, catalase citrate negative, fermenting sugar. Unlike H-Hn, H-Sk able grow at 6.5% NaCl higher temperature (45°C). The antimicrobial properties assessed using agar disk diffusion assay against several indicator organisms (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus Vibrio parahaemolyticus). exhibited varying degree inhibitory organisms. Strain inhibited E. coli V. parahaemolyticus. Meanwhile, showed rather broad-spectrum inhibition. From other L. lactis strain, pathogenic garvieae could also found especially their gut tissues. presence commercial should raise some concern those who like consume this fish. has probiotics potential that commercially used diseases aquaculture.